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Generator

Part:BBa_K1758105

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-27)

mRFP generator under control of T7 promoter

mRFP (BBa_K283035) under control of the T7 promoter (BBa_I719005). This part was used to create (BBa_K1758106.


Usage and Biology

mRFP is synthesized when T7-polymerase is present. This part is a mRFP generator.

Results

This part was used to charactize the effect of our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100), on protein production.

cultures of mRFP producting cells
Image of mRFP producing E. coli cultures. Left: BBa_K1758106, right: This part. Both cultures were cultivated for the same time span. You can see that our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100), improves mRFP production in vivo. See BBa_K1758106 for further details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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